1. Collect the specimen directly into the TesTcup II cup. Check the temperature strip to ensure integrity of the sample. A minimum sample size of 15 mL is required to run the test. Do not use more than 50 mL. Use the volume markings on the cup as a guide.
2. Open the foil pouch and remove the test profile card. The donor's name or ID number may be recorded in the ID area of the card..
3. Insert the profile card into the holder in the lid. To properly orient the card, use the black arrows on the card as a guide; line them up with the arrows on the plastic holder. The printed side of the card should be facing the small "half" of the lid.
4. Place the lid onto the cup with the card facing the flat panel of the cup as shown. Make sure that the card is close to the flat panel and not caught behind either of the cup's two "positioning guides." Firmly secure the lid onto the cup.
5. Allow the test to proceed until Test Valid bands appear. Interpret the results. A blue band indicates a negative result, while the lack of color should be considered a presumptive positive. Faint, broken or partial bands should be interpreted as negative.
Above: Valid bands appear; all drugs test negative.
Below: Valid bands appear; cocaine is positive while all other drugs test negative.
