|
|
|
|
The step-by-step On-Site® OraLab® process
|
|
Collect saliva & start the test
Before the test is administered, instruct the donor to drink 4 ounces
of water, and to place nothing else in the mouth(such as food, drink,
gum, and tobacco products) for 10 minutes prior to collection.
During the minute or two prior to collection, instruct the donor
to draw a pool of saliva into the mouth with a "puckering"
action.
1. Gently open the foil pouch
and remove the contents. Record the donor name and collection date
on the designated area of the test cassette.
2. Collect (and observe) fresh
saliva specimens as follows; no preservative, special handling,
or pretreatment is required.
3. Instruct the donor to open
the collector bag, place the collector foam inside mouth for three
minutes, and completely saturate the collector foam with saliva.
Specifically, instruct that the donor:
• Bathe the collector foam in the pool of saliva
• Alternatively, stimulate salivation by continuously moving
the collection foam along the sides and lower margin of the tongue
• Not chew, compress, or suck on the collector foam
4.
Place the OraLab cassette on a flat surface and lift the confirmation
well cap to assure that the flow of saliva into the well is not
obstructed.
|
 |
 |
5. Remove
the collection foam from the mouth. It should be very wet when removed
from the mouth, and must not be compressed when removed.
• Place the collector foam
vertically into the sample well on top of the device, and push the
collector slowly (approximately 5 seconds) and firmly downward
until it comes to a stop.
• Saliva flows directly into the test cassette.
|
Interpret
test validity and results
Test validity and results must be interpreted between 10 and 15 minutes
after initiating the test.
|
 |
|
Seal the confirmation well 10 minutes after initiating
the test by firmly pushing the cap down. The "click" confirms
that the well is properly sealed. Remove the label covering the
result windows.
|
| |
|
1.
Interpret test validity: A valid test is indicated by a band in
the Test Valid window. Both of the COC/OPI/AMP and MET/PCP/THC reagent
strips pictured below (Figure 3) are valid. Since a valid test may
give a faint or incomplete band, any Test Valid band confirms that
the test is valid. Due to the high viscosity and variability of
some saliva samples, test results may require up to 15 minutes to
form.
An invalid test is indicated by the absence of a distinct band in
the Test Valid window or by a reddish background in the Results
or Test Valid windows which obscures the presence of bands 15 minutes
after the test is initiated. If an invalid result is obtained, see
Step 3 below.
2.
Immediately interpret test results as either negative or preliminary
positive.
|
 |
|
a.
A negative result for a given drug (i.e., drug absent or below the
cutoff) is the presence of a band in the Results window adjacent to
the drug name. The intensity of the bands in the Results windows may
vary. Since a negative sample may give a faint or incomplete band,
any band in the Result window indicates a negative result. The test
results pictured below (Figure 3) are negative for Cocaine, Morphine,
Amphetamine, Methamphetamine, PCP, and THC. |
| |
 |
|
b. A preliminary positive result for a given drug (i.e.,
drug present above the cutoff, suggesting current drug use) is the
absence of a band in the test result window adjacent to the drug
name. The adjacent area appears off-white.
|
c. If all drug results are negative, or if no further
confirmation tests are required, properly discard the test cassette
and collector.
d. When
confirmation of a preliminary positive screen result is required:
• Verify that the volume
of saliva in the confirmation well is sufficient for laboratory
testing by observing the transparent bottom of the confirmation
well. A sufficient specimen will fill at least half of the well.
• Observe any chain of custody procedures in effect at the
collection site.
|
3. If test did not run, if invalid test results are obtained,
or if the confirmation well is less than half full, do not add
additional sample to the same device. Properly discard the original
test cassette and collector. Using a second OraLab device collect
the additional sample by following these instructions:
a.
Collect the second sample within a few minutes
of the first.
b. Instruct the donor to drink 4 ounces of water. Assure
that this water is swallowed and that nothing else is placed in
the mouth for 3-5 minutes.
c. During the minute or two prior
to collection, instruct the donor to draw a pool of saliva into
the mouth with a "puckering" action.
d. Provide a second packaged
collector to the donor, and repeat collection instructions with
emphasis on:
• Bathing the collection foam in saliva(and/or
• Continuous gentle movement of the collector over the tongue
throughout the collection
e. Observe a 4-minute collection
and perform the test according to the instructions in "Collect
Saliva & Start Test"
section.
Test
your knowledge
|
|
|
