Packed Bed: Polymeric
Focus?
Standard Extraction Methods Feature POWER RINSE™ for Cleanest Extracts
Now with Focus you have a simple and effective solution for the extraction of drugs from biological matrices. The following standard protocols can be used as a starting point for any assay. Focus’ unique polar-enhanced sorbent technology uses multiple retention mechanisms to deliver outstanding recovery, cleaner extracts and reduced LC/MS/MS ion-suppression.
Standard Extraction Methods |
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Bases/Neutrals |
Acids |
| Sample Pretreatment |
none |
Add 2% Formic Acid |
| Condition |
1 mL Methanol
1 mL DI Water |
1 mL Methanol
1 mL 2% Formic Acid |
| Apply |
Up to 1 mL Sample |
Up to 1 mL Sample |
| Power Rinse |
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| Elute |
1 mL 6:3:1 MeOH, ACN, Acidified DI Water |
1 mL 6:3:1 MeOH, ACN, 2%NH4OH |
Further Optimization
It is a simple process to further increase the cleanliness of both basic and acidic compound extracts. By altering the pH of the sample load, wash and elution steps, a single 96-well plate can be used to determine the best extraction conditions for an individual analyte. more...
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