Packed Bed: Mixed-Mode
Bond Elut Certify
The Certify extraction cartridge utilizes a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from all manner of aqueous matrices.
|
Recoveries and Precision Data of
Fluoxetine and Norfluoxetine |
|
Concentration
(ng/mL)
|
Fluoxetine
|
Norfluoxetine
|
|
25
50
75
|
%
Recovery |
SD |
%CV |
%
Recovery |
SD |
%CV |
|
|
91
93
97
|
3.06
3.22
2.31
|
3.40
3.50
2.31
|
75
77
80
|
1.53
2.65
4.16
|
2.20
3.40
5.20
|
|
|
|
Average |
94 |
3.06 |
3.30 |
77 |
2.52 |
3.30 | Mean values for Fluoxetine and Norfluoxetine triplicate determinations at each concentration.
Certify may also be used to extract acidic compounds such as the marijuana metabolite, 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid from urine. In this novel approach, the non-polar portion of the sorbent bed is used to retain the analyte while the cationic portion is used to irreversibly retain interferences.
Commonly extracted with Certify are:
- Cocaine and benzoylecgonine
- Amphetamine and methamphetamine
- Phencyclidine
- Opiates
- Barbiturates
- THC metabolite, 11-nor-delta-9- tetrahydrocannabinol-9-carboxylic acid
- Tricyclic antidepressants
- Benzodiazepines
- Anti-psychotics
- Acid/base/neutral screens
The list of drugs extracted with Certify continues to grow. Please refer to the Applications Bibliography for additional compounds or email helpdesk.us@varianinc.com for a copy of the Certify Extraction Manual.
|
| Description of Base Silica: |
40 μm/120 μm irregular acid washed silica, |
|
60 Angstrom mean porosity |
| Bonded functional group: |
Mixed-mode sorbent bed: octyl and benzene |
|
sulfonic acid (SCX) |
| Endcapped: |
No |
| Typical percent carbon loading: |
9.0% |
| Primary retention mechanism: |
Bi-model non-polar and strong cation exchange |
| Typical sample types: |
Urine, biological fluids |
| Illustrative extractions from |
| Applications Bibliography: |
Cocaine and metabolites/Urine, M1123, Drug |
|
screen/Biological fluids, M1809 |
|
 |
|
