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THC Metabolite Extraction
Procedure indicates typical use of SPEC and reduced processing volumes
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SPEC Disc
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Typical Packed-Bed SPE
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Specimen Volume
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2 mL
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3 mL
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Conditioning
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0.15 mL MeOH
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2 mL MeOH 2 mL H3PO4 |
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Sample Application
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-
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-
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Wash
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0.75 mL MeOH
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9 mL H3PO4 3 mL H3PO4/MeOH |
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Adsorbent Drying
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-
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-
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Elution
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0.8 mL hexane/ethyl acetate
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1 mL hexane 1 mL hexane/ethyl acetate |
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Total Solvent Volume
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1.7 mL
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18 mL
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Total Number of Steps
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5
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8
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