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THC Metabolite Extraction Procedure indicates typical use of SPEC and reduced processing volumes
SPEC Disc
Typical Packed-Bed SPE
Specimen Volume
2 mL
3 mL
Conditioning
0.15 mL MeOH
2 mL MeOH
2 mL H3PO4
Sample Application
-
-
Wash
0.75 mL MeOH
9 mL H3PO4
3 mL H3PO4/MeOH
Adsorbent Drying
-
-
Elution
0.8 mL hexane/ethyl acetate
1 mL hexane
1 mL hexane/ethyl acetate
Total Solvent Volume
1.7 mL
18 mL
Total Number of Steps
5
8
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