Liquid/Liquid Extraction
Pharmaceutical Applications
Chem Elut cartridges are ideal for converting labor-intensive liquid/liquid extractions into high-throughput sample processing. Benefits to performing liquid/liquid extractions with Chem Elut are:
- Emulsion-free sample processing
- Fast and automatable
- High reproducibility
Methods are available for a wide variety of analytes (click on Applications link under Support & Resources), or use a general procedure applicable for many moderately polar to nonpolar compounds.
Chem Elut ordering information.
If you’re performing high-throughput bioanalysis with instrumentation such as 96-well liquid handlers and 48-well tube racks, click here for more information about our 96-well Hydromatrix products and tabless Chem Elut cartridges.
Purification Applications
Chem Elut and bulk Hydromatrix are also useful in purification of synthesis mixtures. They are routinely used to scrub reaction mixtures of water-soluble reaction by-products, excess reagents, and impurities.
Find specific purification applications.
High-Throughput Bioanalysis
The benefits of Solid-Supported Liquid/Liquid Extraction (SLE) found in Chem Elut and Hydromatrix products are now available in formats designed for interfacing with high-throughput bioanalytical instrumentation.
Combilute 96-Well Chem Elut Plate
This 96-well version of our popular Chem Elut tubes allows fast processing of bioanalytical samples – 96 wells at a time. Combilute will accommodate up to 500 μL of buffered samples (plasma, serum, urine, and other biofluids) per well. Follow the guidelines for standard SLE (Chem Elut) methods for selecting the appropriate buffer. Then, process the plate as follows:
- Position the Combilute plate on top of a clean 96-well deep-well collection plate secured to your liquid handler’s platform. There is no need for a vacuum source, but manifold bottoms are often helpful to secure the plate in place for processing.
- Dispense buffered sample to each well. There is no need to cap any unused wells.
- Allow 3-5 minutes for sample to adsorb onto the sorbent.
- Elut by dispensing 2 x 1 mL of a water-immiscible solvent to each well. Allow solvent to flow by gravity only.
Tabless tubes
This format of Chem Elut allows close spacing of 3 or 6 mL tubes in a 24 or 48-position tightly packed configuration. Standard tubes (with flanges) cannot be used in these configurations. Tubes are available with up to 1 g Hydromatrix for extracting up to 2 mL biofluid sample.
Order Tabless Chem Elut on line |
