Sample Preparation
Captiva® Filtration
- Faster than Centrifugation
- Easily Automated
- No Plugging
- No Interferences
- No Loss of Analytes or Non-Specific Binding
- Excellent for Sample Storage
- Avoid Fibrinogen Clogging Forever
Plasma Protein Removal
With today’s increasing throughput requirements, you don’t
have time for plugged HPLC columns. Even careful centrifugation
of precipitated plasma proteins leaves particulates that invariably
become trapped on your LC column, causing failure. Now you can save
your HPLC columns by improving your sample prep with Captiva.
Unlike conventional centrifugation,
Captiva filtration eliminates all precipitated proteins and pellets.
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Which sample
would you
rather inject? |
Captiva’s unique dual-depth filtration media provides
both complete removal of precipitated proteins and outstanding
resistance to sample clogging. All Captiva components are
ultra-clean, and rigorously tested to ensure against non-specific
binding. With Captiva your plasma samples are processed
quickly and reliably.
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Captiva Simplifies
Plasma Precipitation
Time-consuming sample transfer steps required with conventional
precipitation are now a thing of the past. With Captiva, clean,
clear filtrates are ready for injection in minutes. And because
Captiva samples are pellet-free, you can sample directly from the
collection plate. Collect, dry-down, and sample directly from one
Captiva Collection Plate—it doesn’t get any easier or
faster!
Captiva is available in packages of five 96-well filter plates:
A5960045 for precipitation using acetonitrile
A5960002 for precipitation using methanol
New to Captiva? Simply order a Starter Kit, containing everything
you need to process 480 samples, including a reusable CaptiVac vacuum
collar:
A5960045SK for precipitation using acetonitrile
Contains:
5 x Captiva 0.45 µm Filter Plates
5 x Captiva 1 mL 96-well Collection Plates
10 x DuoSeal tip & top filter plate sealers
5 x Captiva Collection Plate Covers
1 x CaptiVac vacuum collar
A5960002SK for precipitation using methanol
Contains:
5 x Captiva 0.2 µm Filter Plates
5 x Captiva 1 mL 96-well Collection Plates
10 x DuoSeal tip & top filter plate sealers
5 x Captiva Collection Plate Covers
1 x CaptiVac vacuum collar
Plasma Sample Storage and Fibrinogen
Clot Removal
Frozen plasma samples are notoriously difficult to process after
thawing due to fibrinogen clots. These clots cannot be effectively
centrifuged, and cause plugging of pipette and liquid-handler tips.
The result is failed analysis. Captiva Filter Plates can be used
as plasma sample storage containers which eliminate this problem.
Simply seal the bottom tips of a Captiva 20 µm Filter Plate
(A596002000) with a DuoSeal™ 96
(Order No. A8961008
for package of 10) silicone sealing mat. Next, fill the plate with
96 samples, and seal the top with a second DuoSeal. The samples
are now ready for frozen storage.
When ready for processing, simply remove the bottom DuoSeal, apply
the plate to a CaptiVac vacuum collar
(or other vacuum manifold), and allow to thaw. Then, apply vacuum
to filter and remove clots. Your samples are now clarified and ready
for further processing or analysis.
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