HPLC Columns

Reversed Phase

These materials are hydrophobic (e.g., C18, C8, C4) functional groups bonded to silica particles. Mobile phases are typically water with an organic modifier like methanol or acetonitrile.

Variations in retention behavior of the different reversed phase materials are caused by a variety of parameters such as: surface coverage and chain length. As a general rule, the shorter the hydrocarbon chain, the shorter the retention time. Each of the phases displays a unique selectivity. This means that changing from one C18 phase to another C18 will result changes in elution times and possibly elution order.