TOP: Trityl On Oligonucleotide Purification

A High Throughput, Simple, Cost-Effective Solution for Oligonucleotide Purification

TOP incorporates a unique 96-well plate with removable tubes, streamlined gravity flow procedure and proprietary polymeric resin. Varian's innovative technology delivers superior yield and purity for standard oligos up to 200 nmole in scale and up to 50 mer in length.

Modular 96-Well Plate

• Manually purify 96 oligos in 45 minutes with the full plate option and multi-channel pipettes
• Process multiple plates completely unattended and with improved accuracy and precision using TOP on a robotic system
• Reduce the cost per oligo by 30% with individual tubes and the reusable base plate
• Eliminate wasted wells and cross-contamination associated with fixed 96-well plates

Simple Gravity Flow Procedure

• Generate reproducible yields along with improved sample handling and robot compatibility using gravity flow
• Deliver oligos faster by reducing post-purification dry down time with optimized elution solvent composition and volume
• Minimize start-up costs by eliminating vacuum manifolds and accessories
• Save 70% on disposal and reagent costs and increase throughput on robotic systems by using two-thirds less solvent than other suppliers' products

Proprietary Polymeric Resin and Optimized Binding Buffer

• Save up to 4 hours by eliminating pre-purification evaporation time with direct loading of the deprotection solution
• Improve yields and robot compatibility by eliminating multiple sample loading steps
• Reduce pre-purification time and labor with prepackaged, quality controlled Varian BufferPaks